Flexural Rigidity of Double-stranded DNA as Measured by Electron Microscopy
نویسندگان
چکیده
منابع مشابه
Four-stranded Dna as Determined by Electron Microscopy
Pneumococcus DNA, of weight-average molecular weight 1.6 million by light scattering, had a weight-average length of 4300 A by electron microscopy. Thus, the average mass per unit length was 370 molecular-weight units per A, or approximately two times that expected (208) for a Watson-Crick double helix. This corresponds to an average of 3.6 strands per molecule, which is close to that obtained ...
متن کاملExamination of Lung Parenchyma by Electron Microscopy
In this paper many of the principal structure of the Moral Lung were studied with the help of Electron Microscopy. Detailed description was given of Granular Pneumocytes, Membranous Pneumocytes, Macrophages, and Alveolar Capillaries. A discussion followed on the form and function of Granular Pneumocytes. Acknowledgements I am grateful to the Reza Shah Pahlavi Foundation for its generou...
متن کاملInteraction of lithium and gentamicin in salivary gland by electron microscopy and protein and DNA measurement
The effects of gentamicin, lithium and combination of these drugs on ultrastructural morphology of rat submandibular glands were studied. Daily intraperitoneal injection of 40 mg/kg of gentamicin which causes chronic salivary gland toxicity, led to a marked decrease in gland's dry weight and total protein concentration but did not affect DNA content. Electron micrograph (EM) of submandibular ...
متن کاملSequence dependent rigidity of single stranded DNA.
Single stranded DNA (ssDNA) equilibrium dynamics are investigated using a fluorophore/quencher-labeled hairpin structure which thermally fluctuates between open and closed states. Temporal correlations of the fluorescence fluctuations are used to determine the energy barrier to conformational change. We find that ssDNA distortion is purely entropic for poly(T) but requires an additional enthalp...
متن کاملSequencing double-stranded DNA by strand displacement.
Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides. This allows dsDNA targets to be captured from mixtures and directly sequence...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Polymer Journal
سال: 1970
ISSN: 0032-3896,1349-0540
DOI: 10.1295/polymj.1.457